Hotspot

accD

LSC · 60140-62290 · 2151 bp

Back to Dipelta

Pi 0.0098

MarkerSeek score 57.60

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 2151
variable_sites: 21
pi: 0.0098
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	ACACGACATAGCAGAAATGAGA	CGGGAGGAGGAAATTCGTTT	40.9	50.0	57.5	57.9	2344 bp	0.5	39.5
accD_p2	ACACGACATAGCAGAAATGAGA	TAAGGCGGGAGGAGGAAATT	40.9	50.0	57.5	58.4	2349 bp	0.5	37.1
accD_p3	CGATGTTTAACACGACATAGCA	CGGGAGGAGGAAATTCGTTT	40.9	50.0	57.2	57.9	2353 bp	0.5	36.5
accD_p4	ACACGACATAGCAGAAATGAG	CGGGAGGAGGAAATTCGTTT	42.9	50.0	56.1	57.9	2344 bp	0.5	35.8
accD_p5	ACACGACATAGCAGAAATGAGAT	CGGGAGGAGGAAATTCGTTT	39.1	50.0	58.0	57.9	2344 bp	0.5	35.0