Hotspot

rps12-rps7

IRa · 143489-143541 · 53 bp

Back to Dipelta

Pi 0.0189

MarkerSeek score 63.30

Primer available yes

Label rps12-rps7

Feature Metadata

label_name: rps12-rps7
feature_type: igs
region: IRa
length_bp: 53
variable_sites: 1
pi: 0.0189
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-rps7_p1	GGCTTGGAGGGAGATCTTTC	TGCAGTACCTCTACGTGACA	55.0	50.0	57.7	58.1	143 bp	1.0	77.0
rps12-rps7_p2	ATCGTTCGAGATCCACCCTA	TGCAGTACCTCTACGTGACA	50.0	50.0	57.6	58.1	121 bp	1.0	77.0
rps12-rps7_p3	TATCGTTCGAGATCCACCCT	TGCAGTACCTCTACGTGACA	50.0	50.0	57.6	58.1	122 bp	1.0	77.0
rps12-rps7_p4	TATGTACGGCTTGGAGGGAG	TGCAGTACCTCTACGTGACA	55.0	50.0	58.6	58.1	150 bp	1.0	75.0
rps12-rps7_p5	TCGTTCGAGATCCACCCTAC	TGCAGTACCTCTACGTGACA	55.0	50.0	58.6	58.1	120 bp	1.0	75.0