Hotspot

rpl16-rps3

LSC · 85106-86348 · 1243 bp

Back to Dipelta

Pi 0.0154

MarkerSeek score 68.50

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 1243
variable_sites: 19
pi: 0.0154
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	TCAAGGGCCTGAAGAGCATA	GTTCGAACTATCCATGGGGT	50.0	50.0	58.4	57.0	1419-1425 bp	1.0	70.3
rpl16-rps3_p2	TCAAGGGCCTGAAGAGCATA	CAGTTCGAACTATCCATGGGG	50.0	52.4	58.4	58.2	1421-1427 bp	1.0	69.1
rpl16-rps3_p3	CAAGGGCCTGAAGAGCATAT	GTTCGAACTATCCATGGGGT	50.0	50.0	57.3	57.0	1418-1424 bp	1.0	68.4
rpl16-rps3_p4	TCAAGGGCCTGAAGAGCATA	TCCTCTACAAACCATTCGAGC	50.0	47.6	58.4	57.8	1465-1471 bp	1.0	68.3
rpl16-rps3_p5	TCAAGGGCCTGAAGAGCATA	CCTCTACAAACCATTCGAGCT	50.0	47.6	58.4	57.7	1464-1470 bp	1.0	68.3