Hotspot

clpP-psbB

LSC · 75166-75796 · 631 bp

Back to Dipelta

Pi 0.00173

MarkerSeek score 63.70

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 631
variable_sites: 1
pi: 0.00173
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CTCGCTATCAAGCCAACCAT	ATGGACAGAAAGCAGTCGAC	50.0	50.0	58.0	57.9	790 bp	0.5	59.0
clpP-psbB_p2	TAATTGCACTTCACGCTCCA	ATGGACAGAAAGCAGTCGAC	45.0	50.0	57.8	57.9	721 bp	0.5	57.9
clpP-psbB_p3	CTCGCTATCAAGCCAACCAT	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	741 bp	0.5	57.8
clpP-psbB_p4	CTCGCTATCAAGCCAACCAT	GTCGACCGGGATCATTCAAT	50.0	50.0	58.0	57.8	776 bp	0.5	57.7
clpP-psbB_p5	TTGAGAGCTCGCTATCAAGC	ATGGACAGAAAGCAGTCGAC	50.0	50.0	57.8	57.9	797 bp	0.5	57.6