Hotspot

psbJ-psbL

LSC · 67987-68252 · 266 bp

Back to Dipelta

Pi 0.0494

MarkerSeek score 67.40

Primer available yes

Label psbJ-psbL

Feature Metadata

label_name: psbJ-psbL
feature_type: igs
region: LSC
length_bp: 266
variable_sites: 13
pi: 0.0494
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-psbL_p1	TCCTAGCCTTCGTCACAAGA	ACGACACAATCAAACCCGAA	50.0	45.0	58.1	58.0	1125-1132 bp	1.0	83.2
psbJ-psbL_p2	TCCTAGCCTTCGTCACAAGA	AACTGCCCGTTTTGATCCTT	50.0	45.0	58.1	58.0	1331-1338 bp	1.0	82.9
psbJ-psbL_p3	TCCTAGCCTTCGTCACAAGA	ACTGCCCGTTTTGATCCTTT	50.0	45.0	58.1	58.0	1330-1337 bp	1.0	82.9
psbJ-psbL_p4	TCCTAGCCTTCGTCACAAGA	TCCCTATTCATTGCGGGTTG	50.0	50.0	58.1	57.9	1444-1451 bp	1.0	82.3
psbJ-psbL_p5	TCCTAGCCTTCGTCACAAGA	GCACTGGCTTAGCTTACGAT	50.0	50.0	58.1	58.1	1413-1420 bp	1.0	82.2