Hotspot

psbM-trnD-GUC

LSC · 31032-31900 · 869 bp

Back to Dipelta

Pi 0.00471

MarkerSeek score 63.60

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 869
variable_sites: 4
pi: 0.00471
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAAAGGCGGTAGGGACTAG	TTCAATTGGTCAGAGCACCG	55.0	50.0	58.2	58.5	987-998 bp	1.0	76.3
psbM-trnD-GUC_p2	GAAAGGCGGTAGGGACTAGA	TTCAATTGGTCAGAGCACCG	55.0	50.0	58.2	58.5	986-997 bp	1.0	76.2
psbM-trnD-GUC_p3	AAAGGCGGTAGGGACTAGAA	TTCAATTGGTCAGAGCACCG	50.0	50.0	57.8	58.5	985-996 bp	1.0	76.1
psbM-trnD-GUC_p4	AAGTAGAAAGGCGGTAGGGA	TTCAATTGGTCAGAGCACCG	50.0	50.0	57.8	58.5	991-1002 bp	1.0	76.1
psbM-trnD-GUC_p5	AGAATGAACAGTGCGGTAGC	TTCAATTGGTCAGAGCACCG	50.0	50.0	58.3	58.5	969-980 bp	1.0	75.9