primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	trnS-GCU-trnG-GCC	1	ATTAGCAATCCGCCGCTTTA	TGAATCAAACCGAAAGATCCCT	20	22	45.0	40.9	58.0	57.7	0.0	0.0	2.339278	8904	9685	886-901	1.000000	63.6
trnS-GCU-trnG-GCC_p2	trnS-GCU-trnG-GCC	2	GCTCGTACAACGGATTAGCA	TGAATCAAACCGAAAGATCCCT	20	22	50.0	40.9	58.1	57.7	0.0	0.0	2.383799	8904	9685	899-914	1.000000	63.2
trnS-GCU-trnG-GCC_p3	trnS-GCU-trnG-GCC	3	ACGGAAAGAGAGGGATTCGA	TGAATCAAACCGAAAGATCCCT	20	22	50.0	40.9	58.2	57.7	0.0	0.0	2.452420	8904	9685	935-950	1.000000	62.6
trnS-GCU-trnG-GCC_p4	trnS-GCU-trnG-GCC	4	AGCTCGTACAACGGATTAGC	TGAATCAAACCGAAAGATCCCT	20	22	50.0	40.9	57.8	57.7	0.0	0.0	2.506379	8904	9685	900-915	1.000000	62.2
trnS-GCU-trnG-GCC_p5	trnS-GCU-trnG-GCC	5	TAGTCCACTCAGCCATCTCC	TGAATCAAACCGAAAGATCCCT	20	22	55.0	40.9	58.2	57.7	0.0	0.0	2.519286	8904	9685	868-883	1.000000	62.2
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AGAAAGGCGGTAGGGACTAG	TTCAATTGGTCAGAGCACCG	20	20	55.0	50.0	58.2	58.5	0.0	5.4	0.699391	31032	31900	987-998	1.000000	76.3
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	GAAAGGCGGTAGGGACTAGA	TTCAATTGGTCAGAGCACCG	20	20	55.0	50.0	58.2	58.5	0.0	5.4	0.704495	31032	31900	986-997	1.000000	76.2
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	AAAGGCGGTAGGGACTAGAA	TTCAATTGGTCAGAGCACCG	20	20	50.0	50.0	57.8	58.5	0.0	5.4	0.720214	31032	31900	985-996	1.000000	76.1
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AAGTAGAAAGGCGGTAGGGA	TTCAATTGGTCAGAGCACCG	20	20	50.0	50.0	57.8	58.5	0.0	5.4	0.720214	31032	31900	991-1002	1.000000	76.1
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AGAATGAACAGTGCGGTAGC	TTCAATTGGTCAGAGCACCG	20	20	50.0	50.0	58.3	58.5	0.0	5.4	0.750478	31032	31900	969-980	1.000000	75.9
rbcL-accD_p1	rbcL-accD	1	TAAACAGCTGTCTGGGGGTA	TCAAGGATGGATCTAGGGGG	20	20	50.0	55.0	58.0	57.6	21.9	0.0	0.455904	59582	59701	615-633	1.000000	84.5
rbcL-accD_p2	rbcL-accD	2	CATTCGGAGTTGCGATGAGA	TCAAGGATGGATCTAGGGGG	20	20	50.0	55.0	58.1	57.6	0.0	0.0	0.496378	59582	59701	243-261	1.000000	84.3
rbcL-accD_p3	rbcL-accD	3	GCTGCTTGTGAGGTATGGAA	AAAGAGGACTCCACTTCGGA	20	20	50.0	50.0	57.9	58.0	0.0	8.1	0.124983	58410	60139	2960-2969	1.000000	83.5
rbcL-accD_p4	rbcL-accD	4	GCTGCTTGTGAGGTATGGAA	GGACTCCACTTCGAAATCCC	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.139449	58410	60139	2919-2928	1.000000	83.5
rbcL-accD_p5	rbcL-accD	5	AATGAGGGACGTGATCTTGC	GGACTCCACTTCGAAATCCC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.055427	58410	60139	2994-2994	0.500000	59.6
accD_p1	accD	1	ACACGACATAGCAGAAATGAGA	CGGGAGGAGGAAATTCGTTT	22	20	40.9	50.0	57.5	57.9	0.0	0.0	2.565705	60140	62290	2344-2344	0.500000	39.5
accD_p2	accD	2	ACACGACATAGCAGAAATGAGA	TAAGGCGGGAGGAGGAAATT	22	20	40.9	50.0	57.5	58.4	0.0	0.0	2.864512	60140	62290	2349-2349	0.500000	37.1
accD_p3	accD	3	CGATGTTTAACACGACATAGCA	CGGGAGGAGGAAATTCGTTT	22	20	40.9	50.0	57.2	57.9	0.0	0.0	2.942894	60140	62290	2353-2353	0.500000	36.5
accD_p4	accD	4	ACACGACATAGCAGAAATGAG	CGGGAGGAGGAAATTCGTTT	21	20	42.9	50.0	56.1	57.9	0.0	0.0	3.024094	60140	62290	2344-2344	0.500000	35.8
accD_p5	accD	5	ACACGACATAGCAGAAATGAGAT	CGGGAGGAGGAAATTCGTTT	23	20	39.1	50.0	58.0	57.9	0.0	0.0	3.125475	60140	62290	2344-2344	0.500000	35.0
petA-psbJ_p1	petA-psbJ	1	GGCCTTTTGTTCTTCTTGGC	GGGTCATATTCCGGATTGGG	20	20	50.0	55.0	57.8	57.8	0.0	4.1	0.355226	66860	67866	1133-1142	1.000000	80.0
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTGGC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.8	57.6	0.0	4.1	0.519826	66860	67866	1126-1135	1.000000	78.7
petA-psbJ_p3	petA-psbJ	3	CGTCCAAGGCCTTTTGTTCT	GGGTCATATTCCGGATTGGG	20	20	50.0	55.0	58.7	57.8	17.6	4.1	0.881796	66860	67866	1140-1149	1.000000	75.8
petA-psbJ_p4	petA-psbJ	4	GATCCATTACGCGTCCAAGG	GGGTCATATTCCGGATTGGG	20	20	55.0	55.0	58.8	57.8	18.6	4.1	0.982491	66860	67866	1151-1160	1.000000	75.0
petA-psbJ_p5	petA-psbJ	5	CGTCCAAGGCCTTTTGTTCT	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	58.7	57.6	17.6	4.1	1.046396	66860	67866	1133-1142	1.000000	74.5
psbJ-psbL_p1	psbJ-psbL	1	TCCTAGCCTTCGTCACAAGA	ACGACACAATCAAACCCGAA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.124463	67987	68252	1125-1132	1.000000	83.2
psbJ-psbL_p2	psbJ-psbL	2	TCCTAGCCTTCGTCACAAGA	AACTGCCCGTTTTGATCCTT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.085383	67987	68252	1331-1338	1.000000	82.9
psbJ-psbL_p3	psbJ-psbL	3	TCCTAGCCTTCGTCACAAGA	ACTGCCCGTTTTGATCCTTT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.085383	67987	68252	1330-1337	1.000000	82.9
psbJ-psbL_p4	psbJ-psbL	4	TCCTAGCCTTCGTCACAAGA	TCCCTATTCATTGCGGGTTG	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.132094	67987	68252	1444-1451	1.000000	82.3
psbJ-psbL_p5	psbJ-psbL	5	TCCTAGCCTTCGTCACAAGA	GCACTGGCTTAGCTTACGAT	20	20	50.0	50.0	58.1	58.1	0.0	3.6	0.139435	67987	68252	1413-1420	1.000000	82.2
rps18_p1	rps18	1	CGACCGAAATAGGATTTTTGGG	AATCTGCGTTTGAGTTCGGA	22	20	45.5	45.0	57.7	57.8	7.8	0.0	2.451056	72215	72829	714-720	1.000000	63.5
rps18_p2	rps18	2	CGACCGAAATAGGATTTTTGGG	TCTGCGTTTGAGTTCGGATT	22	20	45.5	45.0	57.7	57.8	7.8	0.0	2.451056	72215	72829	712-718	1.000000	63.5
rps18_p3	rps18	3	CGACCGAAATAGGATTTTTGGG	CAATCTGCGTTTGAGTTCGG	22	20	45.5	50.0	57.7	57.8	7.8	0.0	2.519476	72215	72829	715-721	1.000000	63.0
rps18_p4	rps18	4	CGACCGAAATAGGATTTTTGGG	CTGCGTTTGAGTTCGGATTG	22	20	45.5	50.0	57.7	57.8	7.8	0.0	2.519476	72215	72829	711-717	1.000000	63.0
rps18_p5	rps18	5	CGACCGAAATAGGATTTTTGGG	GAGCAAAACCTCAATCTGCG	22	20	45.5	50.0	57.7	57.7	7.8	0.0	2.540630	72215	72829	726-732	1.000000	62.7
rpl20-rps12_p1	rpl20-rps12	1	CTTGAATGAGCCCCTCGAAA	CAGCGTCGAGGAACATGTAC	20	20	50.0	55.0	57.9	58.7	0.0	9.8	0.844694	73462	74261	894-919	1.000000	75.5
rpl20-rps12_p2	rpl20-rps12	2	TTGAATGAGCCCCTCGAAAG	CAGCGTCGAGGAACATGTAC	20	20	50.0	55.0	57.9	58.7	0.0	9.8	0.844694	73462	74261	893-918	1.000000	75.5
rpl20-rps12_p3	rpl20-rps12	3	CTTGAATGAGCCCCTCGAAA	AGCGTCGAGGAACATGTACT	20	20	50.0	50.0	57.9	58.8	0.0	9.8	0.939077	73462	74261	893-918	1.000000	74.8
rpl20-rps12_p4	rpl20-rps12	4	TTGAATGAGCCCCTCGAAAG	AGCGTCGAGGAACATGTACT	20	20	50.0	50.0	57.9	58.8	0.0	9.8	0.939077	73462	74261	892-917	1.000000	74.8
rpl20-rps12_p5	rpl20-rps12	5	TTGAATGAGCCCCTCGAAAG	TCAGCGTCGAGGAACATGTA	20	20	50.0	50.0	57.9	58.8	0.0	9.8	0.943418	73462	74261	894-919	1.000000	74.7
clpP-psbB_p1	clpP-psbB	1	CTCGCTATCAAGCCAACCAT	ATGGACAGAAAGCAGTCGAC	20	20	50.0	50.0	58.0	57.9	0.0	7.7	0.127013	75166	75796	790-790	0.500000	59.0
clpP-psbB_p2	clpP-psbB	2	TAATTGCACTTCACGCTCCA	ATGGACAGAAAGCAGTCGAC	20	20	45.0	50.0	57.8	57.9	0.0	7.7	0.263448	75166	75796	721-721	0.500000	57.9
clpP-psbB_p3	clpP-psbB	3	CTCGCTATCAAGCCAACCAT	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.278251	75166	75796	741-741	0.500000	57.8
clpP-psbB_p4	clpP-psbB	4	CTCGCTATCAAGCCAACCAT	GTCGACCGGGATCATTCAAT	20	20	50.0	50.0	58.0	57.8	0.0	6.3	0.281452	75166	75796	776-776	0.500000	57.7
clpP-psbB_p5	clpP-psbB	5	TTGAGAGCTCGCTATCAAGC	ATGGACAGAAAGCAGTCGAC	20	20	50.0	50.0	57.8	57.9	21.1	7.7	0.301961	75166	75796	797-797	0.500000	57.6
rpl16_p1	rpl16	1	AGCATATCGGCGATTGCTAG	GACGGAACCTGTGAATCCAA	20	20	50.0	50.0	58.0	57.8	35.6	0.0	0.180724	84695	85105	1245-1258	1.000000	79.6
rpl16_p2	rpl16	2	CATATCGGCGATTGCTAGCT	GACGGAACCTGTGAATCCAA	20	20	50.0	50.0	58.0	57.8	28.9	0.0	0.180724	84695	85105	1243-1256	1.000000	79.6
rpl16_p3	rpl16	3	GAGCATATCGGCGATTGCTA	GACGGAACCTGTGAATCCAA	20	20	50.0	50.0	58.0	57.8	36.0	0.0	0.182620	84695	85105	1246-1259	1.000000	79.6
rpl16_p4	rpl16	4	AGCATATCGGCGATTGCTAG	TCACGTCCGGTTCTGTAGTA	20	20	50.0	50.0	58.0	57.8	35.6	0.0	0.185290	84695	85105	733-733	1.000000	78.7
rpl16_p5	rpl16	5	CATATCGGCGATTGCTAGCT	TCACGTCCGGTTCTGTAGTA	20	20	50.0	50.0	58.0	57.8	28.9	0.0	0.185290	84695	85105	731-731	1.000000	78.7
rpl16-rps3_p1	rpl16-rps3	1	TCAAGGGCCTGAAGAGCATA	GTTCGAACTATCCATGGGGT	20	20	50.0	50.0	58.4	57.0	6.9	21.0	1.418901	85106	86348	1419-1425	1.000000	70.3
rpl16-rps3_p2	rpl16-rps3	2	TCAAGGGCCTGAAGAGCATA	CAGTTCGAACTATCCATGGGG	20	21	50.0	52.4	58.4	58.2	6.9	29.3	1.577709	85106	86348	1421-1427	1.000000	69.1
rpl16-rps3_p3	rpl16-rps3	3	CAAGGGCCTGAAGAGCATAT	GTTCGAACTATCCATGGGGT	20	20	50.0	50.0	57.3	57.0	6.9	21.0	1.655691	85106	86348	1418-1424	1.000000	68.4
rpl16-rps3_p4	rpl16-rps3	4	TCAAGGGCCTGAAGAGCATA	TCCTCTACAAACCATTCGAGC	20	21	50.0	47.6	58.4	57.8	6.9	0.0	1.663450	85106	86348	1465-1471	1.000000	68.3
rpl16-rps3_p5	rpl16-rps3	5	TCAAGGGCCTGAAGAGCATA	CCTCTACAAACCATTCGAGCT	20	21	50.0	47.6	58.4	57.7	6.9	0.0	1.669418	85106	86348	1464-1470	1.000000	68.3
trnI-CAU-ycf2_p1	trnI-CAU-ycf2	1	GTTGGGCGCTTTAACCATTC	GTTGAACCCTGTGAATTGCG	20	20	50.0	50.0	58.0	57.9	7.6	0.0	0.059678	89953	90136	422-447	1.000000	85.2
trnI-CAU-ycf2_p2	trnI-CAU-ycf2	2	ATTGGCATGCATCCAGTAGG	GTTGAACCCTGTGAATTGCG	20	20	50.0	50.0	58.0	57.9	21.4	0.0	0.059356	89953	90136	471-496	1.000000	84.7
trnI-CAU-ycf2_p3	trnI-CAU-ycf2	3	ATTGGCATGCATCCAGTAGG	TATCGTTTCTGGGCCCAATG	20	20	50.0	50.0	58.0	57.9	21.4	28.3	0.060213	89953	90136	626-651	1.000000	83.4
trnI-CAU-ycf2_p4	trnI-CAU-ycf2	4	GTTGGGCGCTTTAACCATTC	CCCTCTTTGTTCCGATCCAG	20	20	50.0	55.0	58.0	58.0	7.6	0.0	0.039039	89953	90136	781-806	1.000000	82.8
trnI-CAU-ycf2_p5	trnI-CAU-ycf2	5	ATTGGCATGCATCCAGTAGG	CCCTCTTTGTTCCGATCCAG	20	20	50.0	55.0	58.0	58.0	21.4	0.0	0.038717	89953	90136	830-855	1.000000	82.6
rps7-rps12_p1	rps7-rps12	1	TGCAGTACCTCTACGTGACA	GGCTTGGAGGGAGATCTTTC	20	20	50.0	55.0	58.1	57.7	0.0	0.0	0.439965	101392	101444	143-143	1.000000	77.0
rps7-rps12_p2	rps7-rps12	2	TGCAGTACCTCTACGTGACA	ATCGTTCGAGATCCACCCTA	20	20	50.0	50.0	58.1	57.6	0.0	0.8	0.459736	101392	101444	121-121	1.000000	77.0
rps7-rps12_p3	rps7-rps12	3	TGCAGTACCTCTACGTGACA	TATCGTTCGAGATCCACCCT	20	20	50.0	50.0	58.1	57.6	0.0	0.8	0.459736	101392	101444	122-122	1.000000	77.0
rps7-rps12_p4	rps7-rps12	4	TGCAGTACCTCTACGTGACA	TATGTACGGCTTGGAGGGAG	20	20	50.0	55.0	58.1	58.6	0.0	0.0	0.689750	101392	101444	150-150	1.000000	75.0
rps7-rps12_p5	rps7-rps12	5	TGCAGTACCTCTACGTGACA	TCGTTCGAGATCCACCCTAC	20	20	50.0	55.0	58.1	58.6	0.0	0.0	0.713221	101392	101444	120-120	1.000000	75.0
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGGATATTGGTCGGCGTTAA	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	57.8	58.0	6.8	0.0	0.251432	116300	117093	899-920	1.000000	80.2
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGGATATTGGTCGGCGTTAA	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	57.8	58.0	6.8	0.0	0.252803	116300	117093	900-921	1.000000	80.2
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGTCGGCGTTAAAAGCTTTG	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.3	58.0	13.5	0.0	0.326006	116300	117093	891-912	1.000000	79.6
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGTCGGCGTTAAAAGCTTTG	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.3	58.0	13.5	0.0	0.327376	116300	117093	892-913	1.000000	79.6
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGGATATTGGTCGGCGTTAA	TCTTTTAGAAGACGGCCTGC	20	20	50.0	50.0	57.8	57.9	6.8	0.0	0.338364	116300	117093	967-988	1.000000	79.3
rps12-rps7_p1	rps12-rps7	1	GGCTTGGAGGGAGATCTTTC	TGCAGTACCTCTACGTGACA	20	20	55.0	50.0	57.7	58.1	0.0	0.0	0.439965	143489	143541	143-143	1.000000	77.0
rps12-rps7_p2	rps12-rps7	2	ATCGTTCGAGATCCACCCTA	TGCAGTACCTCTACGTGACA	20	20	50.0	50.0	57.6	58.1	0.8	0.0	0.459736	143489	143541	121-121	1.000000	77.0
rps12-rps7_p3	rps12-rps7	3	TATCGTTCGAGATCCACCCT	TGCAGTACCTCTACGTGACA	20	20	50.0	50.0	57.6	58.1	0.8	0.0	0.459736	143489	143541	122-122	1.000000	77.0
rps12-rps7_p4	rps12-rps7	4	TATGTACGGCTTGGAGGGAG	TGCAGTACCTCTACGTGACA	20	20	55.0	50.0	58.6	58.1	0.0	0.0	0.689750	143489	143541	150-150	1.000000	75.0
rps12-rps7_p5	rps12-rps7	5	TCGTTCGAGATCCACCCTAC	TGCAGTACCTCTACGTGACA	20	20	55.0	50.0	58.6	58.1	0.0	0.0	0.713221	143489	143541	120-120	1.000000	75.0
ycf2_p1	ycf2	1	GGGAAGTATCATGATCGTCCA	TCCGATTGATTGGTCTGAAGT	21	21	47.6	42.9	57.0	57.0	25.7	0.0	4.010678	152647	152766	314-317	1.000000	49.9
ycf2_p2	ycf2	2	TGGGAAGTATCATGATCGTCC	TCCGATTGATTGGTCTGAAGT	21	21	47.6	42.9	57.0	57.0	12.5	0.0	4.010678	152647	152766	315-318	1.000000	49.9
ycf2_p3	ycf2	3	GGGAAGTATCATGATCGTCCA	TCCGATTGATTGGTCTGAAGTT	21	22	47.6	40.9	57.0	57.7	25.7	0.0	4.315903	152647	152766	314-317	1.000000	47.5
ycf2_p4	ycf2	4	TGGGAAGTATCATGATCGTCC	TCCGATTGATTGGTCTGAAGTT	21	22	47.6	40.9	57.0	57.7	12.5	0.0	4.315903	152647	152766	315-318	1.000000	47.5
ycf2_p5	ycf2	5	GGGAAGTATCATGATCGTCCAA	TCCGATTGATTGGTCTGAAGT	22	21	45.5	42.9	57.7	57.0	25.7	0.0	4.317960	152647	152766	314-317	1.000000	47.5
ycf2-trnI-CAU_p1	ycf2-trnI-CAU	1	GTTGAACCCTGTGAATTGCG	GTTGGGCGCTTTAACCATTC	20	20	50.0	50.0	57.9	58.0	0.0	7.6	0.059678	154797	154980	422-447	1.000000	85.2
ycf2-trnI-CAU_p2	ycf2-trnI-CAU	2	GTTGAACCCTGTGAATTGCG	ATTGGCATGCATCCAGTAGG	20	20	50.0	50.0	57.9	58.0	0.0	21.4	0.059356	154797	154980	471-496	1.000000	84.7
ycf2-trnI-CAU_p3	ycf2-trnI-CAU	3	TATCGTTTCTGGGCCCAATG	ATTGGCATGCATCCAGTAGG	20	20	50.0	50.0	57.9	58.0	28.3	21.4	0.060213	154797	154980	626-651	1.000000	83.4
ycf2-trnI-CAU_p4	ycf2-trnI-CAU	4	CCCTCTTTGTTCCGATCCAG	GTTGGGCGCTTTAACCATTC	20	20	55.0	50.0	58.0	58.0	0.0	7.6	0.039039	154797	154980	781-806	1.000000	82.8
ycf2-trnI-CAU_p5	ycf2-trnI-CAU	5	CCCTCTTTGTTCCGATCCAG	ATTGGCATGCATCCAGTAGG	20	20	55.0	50.0	58.0	58.0	0.0	21.4	0.038717	154797	154980	830-855	1.000000	82.6