Hotspot

ycf1

SSC · 127222-132834 · 5613 bp

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Pi 0.0145

MarkerSeek score 52.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5613
variable_sites: 363
pi: 0.0145
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCGATTGTTGTGGATAGCG	ATGCCGTCACCCATTCTTAC	50.0	50.0	58.1	58.0	1379-1400 bp	1.0	87.2
ycf1_p2	TCCGATTGTTGTGGATAGCG	ACGAAGGGGACTAAACAGGA	50.0	50.0	58.1	58.0	1283-1295 bp	1.0	86.6
ycf1_p3	TCCGATTGTTGTGGATAGCG	ACAGGAACAAGAGGGATCCA	50.0	50.0	58.1	58.0	1269-1281 bp	1.0	86.5
ycf1_p4	CGATGGGGCCCATTTAAGAA	ATGCCGTCACCCATTCTTAC	50.0	50.0	57.9	58.0	815-836 bp	0.823529	78.5
ycf1_p5	CGATGGGGCCCATTTAAGAA	ACGAAGGGGACTAAACAGGA	50.0	50.0	57.9	58.0	725-731 bp	0.823529	77.2