Hotspot

ndhF

SSC · 113351-115606 · 2256 bp

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Pi 0.0104

MarkerSeek score 49.40

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2256
variable_sites: 117
pi: 0.0104
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AAGAGGGATCCACCGAAGAA	TCCGAATCAAGTGCTTTGTT	50.0	40.0	58.0	55.9	2959-2998 bp	1.0	68.4
ndhF_p2	AATCGACCGTTGCGATACAT	TCCGAATCAAGTGCTTTGTT	45.0	40.0	57.8	55.9	2666-2705 bp	1.0	67.4
ndhF_p3	AGGGATCCACCGAAGAAGAT	TCCGAATCAAGTGCTTTGTT	50.0	40.0	57.8	55.9	2956-2995 bp	1.0	67.3
ndhF_p4	ACAGGAACAAGAGGGATCCA	TCCGAATCAAGTGCTTTGTT	50.0	40.0	58.0	55.9	2967-2991 bp	0.882353	63.5
ndhF_p5	ACAGGAACAAGAGGGATCCA	TCCGAATCAAGTGCTTTGTTT	50.0	38.1	58.0	56.6	2967-2991 bp	0.882353	61.6