Hotspot

ndhD

SSC · 118959-120461 · 1503 bp

Back to Diospyros

Pi 0.00656

MarkerSeek score 48.70

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 52
pi: 0.00656
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TTGGACCGTATTCGCGATTT	TCTGCTCCAAGAACAGAGGA	45.0	50.0	58.0	58.0	2154-2179 bp	0.941176	82.0
ndhD_p2	TTGGACCGTATTCGCGATTT	AGATGTGAATCCGCTTGTCC	45.0	50.0	58.0	58.0	2118-2143 bp	0.941176	81.9
ndhD_p3	TTGGACCGTATTCGCGATTT	ATACCTTGGGACGGGTGTAA	45.0	50.0	58.0	58.0	2190-2215 bp	0.941176	81.8
ndhD_p4	TTGGACCGTATTCGCGATTT	GCTATTGGGCTGGCTATTGT	45.0	50.0	58.0	58.0	2590-2649 bp	0.882353	79.6
ndhD_p5	TTACTTGGACCGTATTCGCG	GCTATTGGGCTGGCTATTGT	50.0	50.0	58.1	58.0	2594-2653 bp	0.882353	79.0