Hotspot

rpl32-trnL-UAG

SSC · 116723-117570 · 848 bp

Back to Diospyros

Pi 0.028
MarkerSeek score 75.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
848
variable_sites
103
pi
0.028
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.98
markerseek_score
75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CGACCGGAAATTCAAAAAGT TCCTAAGAGCAGCGTGTCTA 40.0 50.0 54.8 57.9 922-1003 bp 1.0 65.6
rpl32-trnL-UAG_p2 CGACCGGAAATTCAAAAAGT AGCAGCGTGTCTACCAATTT 40.0 45.0 54.8 57.8 915-996 bp 1.0 65.1
rpl32-trnL-UAG_p3 CGACCGGAAATTCAAAAAGT CCTAAGAGCAGCGTGTCTAC 40.0 55.0 54.8 57.8 921-1002 bp 1.0 65.0
rpl32-trnL-UAG_p4 CGACCGGAAATTCAAAAAGT CTAAGAGCAGCGTGTCTACC 40.0 55.0 54.8 57.8 920-1001 bp 1.0 65.0
rpl32-trnL-UAG_p5 CGACCGGAAATTCAAAAAGT GAGCAGCGTGTCTACCAATT 40.0 50.0 54.8 58.3 916-997 bp 1.0 64.4