Hotspot

rps19-rpl2

IRb · 87254-87315 · 62 bp

Back to Diospyros

Pi 0.0601

MarkerSeek score 76.90

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 62
variable_sites: 10
pi: 0.0601
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	TCTGCTTTTGTGTTAAGCTT	TTGATTCTTCGTCGCCGTAG	35.0	50.0	53.6	58.1	156-169 bp	1.0	54.6
rps19-rpl2_p2	TTCTGCTTTTGTGTTAAGCT	TTGATTCTTCGTCGCCGTAG	35.0	50.0	53.6	58.1	157-170 bp	1.0	54.6
rps19-rpl2_p3	TCTGCTTTTGTGTTAAGCTT	GGGCCCCAATTGGTAGAAAA	35.0	50.0	53.6	58.1	248-261 bp	1.0	52.7
rps19-rpl2_p4	TTCTGCTTTTGTGTTAAGCT	GGGCCCCAATTGGTAGAAAA	35.0	50.0	53.6	58.1	249-262 bp	1.0	52.6
rps19-rpl2_p5	TCTGCTTTTGTGTTAAGCTT	CACAACCCCTTGGGGTTATC	35.0	55.0	53.6	58.2	223-236 bp	1.0	52.3