Pi
0.0224
MarkerSeek score
65.80
Primer available
yes
Label
accD-psaI
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| accD-psaI_p1 | TTCTTTCCTTTGAATCAAAATTC | TTGCCATTGCCGGAAATACT | 26.1 | 45.0 | 52.1 | 58.2 | 872-913 bp | 1.0 | 50.2 |
| accD-psaI_p2 | TTCTTTCCTTTGAATCAAAATTC | TGCCATTGCCGGAAATACTA | 26.1 | 45.0 | 52.1 | 57.3 | 871-912 bp | 1.0 | 50.2 |
| accD-psaI_p3 | TTCTTTCCTTTGAATCAAAATTC | CATTGCCATTGCCGGAAATA | 26.1 | 45.0 | 52.1 | 57.1 | 874-915 bp | 1.0 | 50.2 |
| accD-psaI_p4 | TTCTTTCCTTTGAATCAAAATTC | AAAGAAGCCATTGCCATTGC | 26.1 | 45.0 | 52.1 | 57.9 | 882-923 bp | 1.0 | 50.1 |
| accD-psaI_p5 | TTCTTTCCTTTGAATCAAAATTC | GCCGGAAATACTAGGCCTAC | 26.1 | 55.0 | 52.1 | 57.5 | 864-905 bp | 1.0 | 50.1 |