Hotspot

trnG-GCC-trnfM-CAU

LSC · 38492-38632 · 141 bp

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Pi 0.0764
MarkerSeek score 80.80
Primer available yes
Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name
trnG-GCC-trnfM-CAU
feature_type
igs
region
LSC
length_bp
141
variable_sites
35
pi
0.0764
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.99
markerseek_score
80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnfM-CAU_p1 ATGCGGATATGGTCGAATGG GGGTAGAGCAGTTTGGTAGC 50.0 55.0 57.9 58.0 275-303 bp 1.0 91.0
trnG-GCC-trnfM-CAU_p2 ATGCGGATATGGTCGAATGG CTTGAGGTCACGGGTTCAAA 50.0 50.0 57.9 58.0 239-267 bp 1.0 90.9
trnG-GCC-trnfM-CAU_p3 TCTTTGCCAAGGAGAAGACG GGGTAGAGCAGTTTGGTAGC 50.0 55.0 57.8 58.0 246-274 bp 1.0 90.4
trnG-GCC-trnfM-CAU_p4 ATGCGGATATGGTCGAATGG GTCACGGGTTCAAATCCTGT 50.0 50.0 57.9 58.1 233-261 bp 1.0 90.3
trnG-GCC-trnfM-CAU_p5 TCTTTGCCAAGGAGAAGACG CTTGAGGTCACGGGTTCAAA 50.0 50.0 57.8 58.0 210-238 bp 1.0 90.3