Hotspot

psbZ-trnG-GCC

LSC · 38148-38420 · 273 bp

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Pi 0.0127
MarkerSeek score 55.70
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
273
variable_sites
25
pi
0.0127
unique_haplotype_count
15
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 TCCTTAATTCTCTCATCTCTTGA CGTCTTCTCCTTGGCAAAGA 34.8 50.0 54.3 57.8 340-349 bp 1.0 47.7
psbZ-trnG-GCC_p2 ATCCTTAATTCTCTCATCTCTTGA CGTCTTCTCCTTGGCAAAGA 33.3 50.0 54.8 57.8 341-350 bp 1.0 47.7
psbZ-trnG-GCC_p3 GGTATCCTTAATTCTCTCATCTCT CGTCTTCTCCTTGGCAAAGA 37.5 50.0 55.0 57.8 344-353 bp 1.0 47.6
psbZ-trnG-GCC_p4 GGTATCCTTAATTCTCTCATCTCTTG CGTCTTCTCCTTGGCAAAGA 38.5 50.0 56.9 57.8 344-353 bp 1.0 47.6
psbZ-trnG-GCC_p5 GGTATCCTTAATTCTCTCATCTCTTGA CGTCTTCTCCTTGGCAAAGA 37.0 50.0 58.1 57.8 344-353 bp 1.0 47.6