Hotspot

trnS-GCU-trnG-UCC

LSC · 9416-10064 · 649 bp

Back to Diospyros

Pi 0.0152

MarkerSeek score 68.70

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 649
variable_sites: 39
pi: 0.0152
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	AACCGAAAGACCCCTTAACT	45.0	45.0	58.0	56.4	749-777 bp	1.0	74.2
trnS-GCU-trnG-UCC_p2	ACGGAAAGAGAGGGATTCGA	AACCGAAAGACCCCTTAACT	50.0	45.0	58.2	56.4	798-826 bp	1.0	72.9
trnS-GCU-trnG-UCC_p3	ATTAGCAATCCGCCGCTTTA	AAACCGAAAGACCCCTTAACT	45.0	42.9	58.0	57.1	750-778 bp	1.0	72.3
trnS-GCU-trnG-UCC_p4	GCTTTAGTCCACTCAGCCAT	AACCGAAAGACCCCTTAACT	50.0	45.0	57.6	56.4	735-763 bp	1.0	71.3
trnS-GCU-trnG-UCC_p5	ACGGAAAGAGAGGGATTCGA	AAACCGAAAGACCCCTTAACT	50.0	42.9	58.2	57.1	799-827 bp	1.0	71.0