Hotspot

trnH-GUG-psbA

LSC · 93-547 · 455 bp

Back to Diospyros

Pi 0.0348

MarkerSeek score 69.10

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 455
variable_sites: 63
pi: 0.0348
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	TCAGTGCTATGCATGGTTCC	50.0	50.0	57.9	58.0	710-1077 bp	1.0	89.5
trnH-GUG-psbA_p2	CTGCCTTAATCCACTTGGCT	CAGTGCTATGCATGGTTCCT	50.0	50.0	57.9	58.0	709-1076 bp	1.0	89.5
trnH-GUG-psbA_p3	ACTGCCTTAATCCACTTGGC	TCAGTGCTATGCATGGTTCC	50.0	50.0	58.2	58.0	711-1078 bp	1.0	89.3
trnH-GUG-psbA_p4	CTGCCTTAATCCACTTGGCT	GGTGTAGCTGGTGTATTCGG	50.0	55.0	57.9	58.1	741-1108 bp	1.0	89.1
trnH-GUG-psbA_p5	CTGCCTTAATCCACTTGGCT	ATGCACCCATTCCACATGTT	50.0	45.0	57.9	58.1	762-1129 bp	1.0	89.0