Hotspot

ycf1

SSC · 107919-113507 · 5589 bp

Back to Dioscorea

Pi 0.0316

MarkerSeek score 64.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5589
variable_sites: 840
pi: 0.0316
unique_haplotype_count: 38
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCTCGGGTTATGGAAGAAGG	GCTTTTCTTGAACGAAGCCC	55.0	50.0	58.0	57.9	91-2278 bp	0.809524	83.0
ycf1_p2	GCTCGGGTTATGGAAGAAGG	TCTGGTTCTCTTCTCAGCCA	55.0	50.0	58.0	58.0	91-2278 bp	0.666667	75.8
ycf1_p3	AGGGCCCTCTTATCTCTTCC	TCTGGTTCTCTTCTCAGCCA	55.0	50.0	57.9	58.0	92-2278 bp	0.666667	75.2
ycf1_p4	GGGCCCTCTTATCTCTTCCT	TCTGGTTCTCTTCTCAGCCA	55.0	50.0	57.9	58.0	91-2278 bp	0.666667	75.2
ycf1_p5	AAGGGGGAGAAAGTGAGGAA	TCTGGTTCTCTTCTCAGCCA	50.0	50.0	57.9	58.0	93-2279 bp	0.666667	74.7