Hotspot

ndhF

SSC · 124757-126994 · 2238 bp

Back to Dioscorea

Pi 0.0262

MarkerSeek score 67.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2238
variable_sites: 272
pi: 0.0262
unique_haplotype_count: 36
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AATCCGCACAATTTCCCCTT	GAGCTACTGAGGAACAACGG	45.0	55.0	58.1	58.0	93-2281 bp	0.928571	88.6
ndhF_p2	ACAGCAGGATTAACCGCATT	GCTTCCTTCGTAACTCTCGG	45.0	55.0	57.9	58.1	93-2280 bp	0.547619	69.2
ndhF_p3	ACTTTTGATGGCTGTTTGCG	GCTTCCTTCGTAACTCTCGG	45.0	55.0	57.9	58.1	92-2278 bp	0.547619	69.2
ndhF_p4	GTATGGGGGAGAGAACCACT	GCTTCCTTCGTAACTCTCGG	55.0	55.0	58.1	58.1	91-2280 bp	0.52381	68.4
ndhF_p5	CTGTATGGGGGAGAGAACCA	GCTTCCTTCGTAACTCTCGG	55.0	55.0	58.1	58.1	91-2716 bp	0.52381	68.1