Hotspot

atpF

LSC · 10801-12343 · 1543 bp

Back to Dioscorea

Pi 0.0305

MarkerSeek score 70.80

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1543
variable_sites: 229
pi: 0.0305
unique_haplotype_count: 36
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	ACGAGCAATACCATCCCCTA	GTGGCATTGGCGCTTTTATT	50.0	45.0	57.9	58.0	969-2277 bp	0.97619	90.2
atpF_p2	CTAATTCGCCTGCCATCACT	GTGGCATTGGCGCTTTTATT	50.0	45.0	58.0	58.0	1006-2279 bp	0.952381	89.7
atpF_p3	CTAATTCGCCTGCCATCACT	AAGCCGTAGAAGGTATTGCG	50.0	50.0	58.0	58.1	1008-2373 bp	0.952381	89.3
atpF_p4	GAGCAATGCCTACTGTACCC	GTGGCATTGGCGCTTTTATT	55.0	45.0	58.0	58.0	1039-2294 bp	0.880952	86.8
atpF_p5	GCAATGCCTACTGTACCCTC	GTGGCATTGGCGCTTTTATT	55.0	45.0	58.0	58.0	1037-2292 bp	0.880952	86.8