Hotspot

ndhC-trnV-UAC

LSC · 49841-50826 · 986 bp

Back to Dioscorea

Pi 0.0416
MarkerSeek score 78.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
986
variable_sites
185
pi
0.0416
unique_haplotype_count
34
primer_available
yes
alignment_reliability
0.95
markerseek_score
78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGCCAAGATAGGAATAATGCTTG GGTTCGAATCCGTATAGCCC 39.1 55.0 57.0 57.9 336-2279 bp 1.0 59.1
ndhC-trnV-UAC_p2 TGCCAAGATAGGAATAATGCT GGTTCGAATCCGTATAGCCC 38.1 55.0 54.7 57.9 336-2279 bp 1.0 57.3
ndhC-trnV-UAC_p3 TGCCAAGATAGGAATAATGCTTG CGGTTCGAATCCGTATAGCC 39.1 55.0 57.0 58.3 336-2279 bp 1.0 57.3
ndhC-trnV-UAC_p4 GCCAAGATAGGAATAATGCTTGA GGTTCGAATCCGTATAGCCC 39.1 55.0 56.7 57.9 335-2278 bp 1.0 57.1
ndhC-trnV-UAC_p5 TGCCAAGATAGGAATAATGCTTGA GGTTCGAATCCGTATAGCCC 37.5 55.0 58.3 57.9 336-2279 bp 1.0 56.9