Hotspot

trnF-GAA-ndhJ

LSC · 47430-48118 · 689 bp

Back to Dioscorea

Pi 0.0402

MarkerSeek score 76.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 689
variable_sites: 172
pi: 0.0402
unique_haplotype_count: 34
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAATACAAGATGCTCGTTGA	50.0	36.4	58.1	56.1	620-2280 bp	0.97619	59.5
trnF-GAA-ndhJ_p2	AATCCTCGTGTCACCAGTTC	TGAAATACAAGATGCTCGTTGA	50.0	36.4	57.5	56.1	623-2283 bp	0.97619	56.3
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TGAAATACAAGATGCTCGTTG	50.0	38.1	58.1	54.6	620-2279 bp	0.97619	55.5
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	ATGAAATACAAGATGCTCGTTGA	50.0	34.8	58.1	56.6	621-2281 bp	0.97619	55.5
trnF-GAA-ndhJ_p5	TGGTAGAGCAGAGGACTGAA	TGAAATACAAGATGCTCGTTGA	50.0	36.4	57.4	56.1	643-2281 bp	0.97619	55.1