Hotspot

rpoB-trnC-GCA

LSC · 25930-27200 · 1271 bp

Back to Dioscorea

Pi 0.0386

MarkerSeek score 82.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1271
variable_sites: 226
pi: 0.0386
unique_haplotype_count: 39
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCCCTCATTTCCATCCCGAA	TTAGGCGACACCCAGATTTG	50.0	50.0	58.4	57.9	900-2279 bp	0.97619	86.9
rpoB-trnC-GCA_p2	TCCCTCATTTCCATCCCGAA	TTGTTGATTAGGCGACACCC	50.0	50.0	58.4	58.2	900-2279 bp	0.97619	86.3
rpoB-trnC-GCA_p3	GTTCTTCCATCAAGCCCTGA	TTAGGCGACACCCAGATTTG	50.0	50.0	57.5	57.9	979-2278 bp	0.97619	86.2
rpoB-trnC-GCA_p4	TTCCCTCATTTCCATCCCGA	TTAGGCGACACCCAGATTTG	50.0	50.0	58.4	57.9	901-2279 bp	0.952381	86.0
rpoB-trnC-GCA_p5	TTCCCTCATTTCCATCCCGA	TTGTTGATTAGGCGACACCC	50.0	50.0	58.4	58.2	901-2279 bp	0.952381	85.3