Hotspot

atpH-atpI

LSC · 12847-13644 · 798 bp

Back to Dioscorea

Pi 0.0366

MarkerSeek score 72.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 798
variable_sites: 134
pi: 0.0366
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	CGCAATACCTTCTACGGCTT	AGCAAAGCCCAAATTGGAGA	50.0	45.0	58.1	58.0	1297-2571 bp	1.0	91.4
atpH-atpI_p2	AATAAAAGCGCCAATGCCAC	AGCAAAGCCCAAATTGGAGA	45.0	45.0	58.0	58.0	1404-2573 bp	0.97619	90.9
atpH-atpI_p3	AATAAAAGCGCCAATGCCAC	TATGGTCCTTGGGTTCCCTT	45.0	50.0	58.0	57.9	1380-2549 bp	0.97619	90.4
atpH-atpI_p4	AATAAAAGCGCCAATGCCAC	CTGCGCCCACGAATGATATA	45.0	50.0	58.0	57.9	1268-2437 bp	0.97619	90.3
atpH-atpI_p5	AATAAAAGCGCCAATGCCAC	GCGAATCCATGGAAGGTCAT	45.0	50.0	58.0	58.0	932-2280 bp	0.952381	89.8