Hotspot

trnK-UUU-trnQ-UUG

LSC · 4026-5642 · 1617 bp

Back to Dioscorea

Pi 0.0452
MarkerSeek score 80.50
Primer available yes
Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name
trnK-UUU-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1617
variable_sites
376
pi
0.0452
unique_haplotype_count
38
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-trnQ-UUG_p1 AAAGCCGAGTACTCTACCGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 976-2657 bp 1.0 90.0
trnK-UUU-trnQ-UUG_p2 AAGCCGAGTACTCTACCGTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 975-2656 bp 1.0 90.0
trnK-UUU-trnQ-UUG_p3 AAAGCCGAGTACTCTACCGT CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.2 57.8 976-2661 bp 1.0 89.0
trnK-UUU-trnQ-UUG_p4 AAGCCGAGTACTCTACCGTT CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.2 57.8 975-2660 bp 1.0 89.0
trnK-UUU-trnQ-UUG_p5 TACCGTTGAGTTAGCAACCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.5 58.1 962-2643 bp 1.0 87.7