primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-trnQ-UUG_p1	trnK-UUU-trnQ-UUG	1	AAAGCCGAGTACTCTACCGT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	20.3	16.9	0.244521	4026	5642	976-2657	1.000000	90.0
trnK-UUU-trnQ-UUG_p2	trnK-UUU-trnQ-UUG	2	AAGCCGAGTACTCTACCGTT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	20.3	16.9	0.244521	4026	5642	975-2656	1.000000	90.0
trnK-UUU-trnQ-UUG_p3	trnK-UUU-trnQ-UUG	3	AAAGCCGAGTACTCTACCGT	CTCGGAGGTTCGAATCCTTC	20	20	50.0	55.0	58.2	57.8	20.3	16.9	0.379772	4026	5642	976-2661	1.000000	89.0
trnK-UUU-trnQ-UUG_p4	trnK-UUU-trnQ-UUG	4	AAGCCGAGTACTCTACCGTT	CTCGGAGGTTCGAATCCTTC	20	20	50.0	55.0	58.2	57.8	20.3	16.9	0.379772	4026	5642	975-2660	1.000000	89.0
trnK-UUU-trnQ-UUG_p5	trnK-UUU-trnQ-UUG	5	TACCGTTGAGTTAGCAACCC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.5	58.1	0.0	16.9	0.536858	4026	5642	962-2643	1.000000	87.7
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CCGTCGCTTTAATCCACTCA	ACGAATCGCACTTTTACCACT	20	21	50.0	42.9	58.0	58.2	0.0	1.2	1.241837	7012	8027	358-2278	1.000000	82.1
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	CCGTCGCTTTAATCCACTCA	ACGAATCGCACTTTTACCAC	20	20	50.0	45.0	58.0	56.8	0.0	1.2	1.248863	7012	8027	357-2278	1.000000	82.0
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	TCGCTTTAATCCACTCAGCC	ACGAATCGCACTTTTACCACT	20	21	50.0	42.9	58.0	58.2	0.0	1.2	1.261430	7012	8027	355-2279	1.000000	81.9
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	TCGCTTTAATCCACTCAGCC	ACGAATCGCACTTTTACCAC	20	20	50.0	45.0	58.0	56.8	0.0	1.2	1.268456	7012	8027	354-2279	1.000000	81.9
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	CAGATTAGCAATCCGTCGCT	ACGAATCGCACTTTTACCACT	20	21	50.0	42.9	58.1	58.2	5.6	1.2	1.379163	7012	8027	370-2279	1.000000	81.0
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.081443	8866	9012	220-2279	1.000000	73.6
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.1	56.9	0.0	0.0	2.110280	8866	9012	223-2279	1.000000	73.3
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.164771	8866	9012	226-2279	1.000000	72.8
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	20	22	55.0	45.5	58.1	57.9	0.0	0.0	2.193608	8866	9012	229-2279	1.000000	72.5
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	57.7	56.9	0.0	0.0	2.385293	8866	9012	224-2279	1.000000	71.1
atpF_p1	atpF	1	ACGAGCAATACCATCCCCTA	GTGGCATTGGCGCTTTTATT	20	20	50.0	45.0	57.9	58.0	0.0	15.8	0.105862	10801	12343	969-2277	0.976190	90.2
atpF_p2	atpF	2	CTAATTCGCCTGCCATCACT	GTGGCATTGGCGCTTTTATT	20	20	50.0	45.0	58.0	58.0	0.0	15.8	0.049710	10801	12343	1006-2279	0.952381	89.7
atpF_p3	atpF	3	CTAATTCGCCTGCCATCACT	AAGCCGTAGAAGGTATTGCG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.105026	10801	12343	1008-2373	0.952381	89.3
atpF_p4	atpF	4	GAGCAATGCCTACTGTACCC	GTGGCATTGGCGCTTTTATT	20	20	55.0	45.0	58.0	58.0	0.0	15.8	0.053825	10801	12343	1039-2294	0.880952	86.8
atpF_p5	atpF	5	GCAATGCCTACTGTACCCTC	GTGGCATTGGCGCTTTTATT	20	20	55.0	45.0	58.0	58.0	0.0	15.8	0.053825	10801	12343	1037-2292	0.880952	86.8
atpH-atpI_p1	atpH-atpI	1	CGCAATACCTTCTACGGCTT	AGCAAAGCCCAAATTGGAGA	20	20	50.0	45.0	58.1	58.0	0.0	14.6	0.078077	12847	13644	1297-2571	1.000000	91.4
atpH-atpI_p2	atpH-atpI	2	AATAAAAGCGCCAATGCCAC	AGCAAAGCCCAAATTGGAGA	20	20	45.0	45.0	58.0	58.0	2.3	14.6	0.022761	12847	13644	1404-2573	0.976190	90.9
atpH-atpI_p3	atpH-atpI	3	AATAAAAGCGCCAATGCCAC	TATGGTCCTTGGGTTCCCTT	20	20	45.0	50.0	58.0	57.9	2.3	9.4	0.077583	12847	13644	1380-2549	0.976190	90.4
atpH-atpI_p4	atpH-atpI	4	AATAAAAGCGCCAATGCCAC	CTGCGCCCACGAATGATATA	20	20	45.0	50.0	58.0	57.9	2.3	4.2	0.087600	12847	13644	1268-2437	0.976190	90.3
atpH-atpI_p5	atpH-atpI	5	AATAAAAGCGCCAATGCCAC	GCGAATCCATGGAAGGTCAT	20	20	45.0	50.0	58.0	58.0	2.3	20.7	0.035880	12847	13644	932-2280	0.952381	89.8
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	TCCCTCATTTCCATCCCGAA	TTAGGCGACACCCAGATTTG	20	20	50.0	50.0	58.4	57.9	0.0	0.0	0.517359	25930	27200	900-2279	0.976190	86.9
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	TCCCTCATTTCCATCCCGAA	TTGTTGATTAGGCGACACCC	20	20	50.0	50.0	58.4	58.2	0.0	0.0	0.593335	25930	27200	900-2279	0.976190	86.3
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	GTTCTTCCATCAAGCCCTGA	TTAGGCGACACCCAGATTTG	20	20	50.0	50.0	57.5	57.9	0.0	0.0	0.604848	25930	27200	979-2278	0.976190	86.2
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	TTCCCTCATTTCCATCCCGA	TTAGGCGACACCCAGATTTG	20	20	50.0	50.0	58.4	57.9	0.0	0.0	0.517359	25930	27200	901-2279	0.952381	86.0
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	TTCCCTCATTTCCATCCCGA	TTGTTGATTAGGCGACACCC	20	20	50.0	50.0	58.4	58.2	0.0	0.0	0.593335	25930	27200	901-2279	0.952381	85.3
trnC-GCA-petN_p1	trnC-GCA-petN	1	CAAATCTGGGTGTCGCCTAA	TACCCCTAGAGTCCACTCCT	20	20	50.0	55.0	57.9	58.0	1.7	3.0	0.110610	27282	28227	652-2278	0.976190	90.2
trnC-GCA-petN_p2	trnC-GCA-petN	2	GGGTGTCGCCTAATCAACAA	TACCCCTAGAGTCCACTCCT	20	20	50.0	55.0	58.2	58.0	1.7	3.0	0.186585	27282	28227	645-2277	0.976190	89.6
trnC-GCA-petN_p3	trnC-GCA-petN	3	CAAATCTGGGTGTCGCCTAA	AGTCCACTCCTTCCCCATAC	20	20	50.0	55.0	57.9	58.1	1.7	0.0	0.227009	27282	28227	653-2279	0.976190	89.2
trnC-GCA-petN_p4	trnC-GCA-petN	4	CAAATCTGGGTGTCGCCTAA	GTCCACTCCTTCCCCATACT	20	20	50.0	55.0	57.9	58.1	1.7	0.0	0.227009	27282	28227	652-2278	0.976190	89.2
trnC-GCA-petN_p5	trnC-GCA-petN	5	CAAATCTGGGTGTCGCCTAA	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	57.9	58.2	1.7	0.0	0.259298	27282	28227	654-2277	0.976190	89.0
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	ATTGATTCTTTCGACGGCCA	AGTTCAATCGGTCAGAGCAC	20	20	45.0	50.0	57.9	57.9	2.2	0.0	0.194004	29227	30140	1496-2306	0.642857	71.7
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	ATTGATTCTTTCGACGGCCA	TCTCTTTCAAGGAGGCAACG	20	20	45.0	50.0	57.9	57.8	2.2	0.0	0.277782	29227	30140	2054-2858	0.642857	71.4
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	TTGATTCTTTCGACGGCCAT	AGTTCAATCGGTCAGAGCAC	20	20	45.0	50.0	57.9	57.9	2.2	0.0	0.194004	29227	30140	1495-2305	0.571429	68.8
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	TTGATTCTTTCGACGGCCAT	TCTCTTTCAAGGAGGCAACG	20	20	45.0	50.0	57.9	57.8	2.2	0.0	0.277782	29227	30140	2053-2857	0.571429	68.4
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	TTGATTCTTTCGACGGCCAT	ATAGACCTCACCGGGATTGT	20	20	45.0	50.0	57.9	57.8	2.2	6.3	0.284197	29227	30140	1515-2325	0.571429	68.1
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	45731	46587	861-2278	1.000000	90.9
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GAGTCGAACCGATGACCATC	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	58.2	58.0	0.0	0.0	0.163174	45731	46587	892-2279	1.000000	90.7
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	45731	46587	869-2278	1.000000	90.4
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GAGTCGAACCGATGACCATC	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	58.2	58.1	0.0	0.0	0.227983	45731	46587	900-2279	1.000000	90.2
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	20	20	55.0	50.0	57.9	58.2	0.0	10.2	0.372548	45731	46587	875-2277	1.000000	89.0
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	TGAAATACAAGATGCTCGTTGA	20	22	50.0	36.4	58.1	56.1	0.0	0.4	3.938560	47430	48118	620-2280	0.976190	59.5
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	AATCCTCGTGTCACCAGTTC	TGAAATACAAGATGCTCGTTGA	20	22	50.0	36.4	57.5	56.1	0.0	0.4	4.344030	47430	48118	623-2283	0.976190	56.3
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CCTCGTGTCACCAGTTCAAA	TGAAATACAAGATGCTCGTTG	20	21	50.0	38.1	58.1	54.6	0.0	0.0	4.440150	47430	48118	620-2279	0.976190	55.5
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CCTCGTGTCACCAGTTCAAA	ATGAAATACAAGATGCTCGTTGA	20	23	50.0	34.8	58.1	56.6	0.0	0.4	4.442509	47430	48118	621-2281	0.976190	55.5
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	TGGTAGAGCAGAGGACTGAA	TGAAATACAAGATGCTCGTTGA	20	22	50.0	36.4	57.4	56.1	0.0	0.4	4.491576	47430	48118	643-2281	0.976190	55.1
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TGCCAAGATAGGAATAATGCTTG	GGTTCGAATCCGTATAGCCC	23	20	39.1	55.0	57.0	57.9	0.0	0.0	4.108723	49841	50826	336-2279	1.000000	59.1
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TGCCAAGATAGGAATAATGCT	GGTTCGAATCCGTATAGCCC	21	20	38.1	55.0	54.7	57.9	0.0	0.0	4.337598	49841	50826	336-2279	1.000000	57.3
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TGCCAAGATAGGAATAATGCTTG	CGGTTCGAATCCGTATAGCC	23	20	39.1	55.0	57.0	58.3	0.0	22.8	4.338104	49841	50826	336-2279	1.000000	57.3
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	GCCAAGATAGGAATAATGCTTGA	GGTTCGAATCCGTATAGCCC	23	20	39.1	55.0	56.7	57.9	0.0	0.0	4.364099	49841	50826	335-2278	1.000000	57.1
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TGCCAAGATAGGAATAATGCTTGA	GGTTCGAATCCGTATAGCCC	24	20	37.5	55.0	58.3	57.9	0.0	0.0	4.382018	49841	50826	336-2279	1.000000	56.9
petA-psbJ_p1	petA-psbJ	1	GGCATCTGTGATTTTGGCAC	GGGTTGGGTTCATCTCTGTAG	20	21	50.0	52.4	58.0	57.7	0.0	0.0	1.268452	62242	63132	92-2279	1.000000	81.9
petA-psbJ_p2	petA-psbJ	2	GGCATCTGTGATTTTGGCAC	GGGTTGGGTTCATCTCTGTA	20	20	50.0	50.0	58.0	56.5	0.0	0.0	1.467602	62242	63132	91-2276	1.000000	80.3
petA-psbJ_p3	petA-psbJ	3	TGGCATCTGTGATTTTGGCA	GGGTTGGGTTCATCTCTGTAG	20	21	45.0	52.4	58.4	57.7	0.0	0.0	1.637800	62242	63132	92-2280	1.000000	78.9
petA-psbJ_p4	petA-psbJ	4	TGGCATCTGTGATTTTGGCA	GGGTTGGGTTCATCTCTGTA	20	20	45.0	50.0	58.4	56.5	0.0	0.0	1.836950	62242	63132	91-2277	1.000000	77.3
petA-psbJ_p5	petA-psbJ	5	GCATCTGTGATTTTGGCACA	GGGTTGGGTTCATCTCTGTAG	20	21	45.0	52.4	57.3	57.7	0.0	0.0	1.996712	62242	63132	92-2279	1.000000	76.0
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	CGAACTGAACTAAGAGCGCT	GCTTTCGAACCAGCATCCTA	20	20	50.0	50.0	58.0	58.0	11.3	15.3	0.040203	65382	65758	91-2276	0.976190	90.7
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	CGAACTGAACTAAGAGCGCT	CTGACATCCTTACCCTTGGC	20	20	50.0	55.0	58.0	58.0	11.3	0.0	0.060150	65382	65758	91-2278	0.976190	90.6
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	TATTTCTCCGGAATTGCGGG	GCTTTCGAACCAGCATCCTA	20	20	50.0	50.0	58.0	58.0	34.0	15.3	0.062382	65382	65758	92-2277	0.619048	74.7
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	CGAACTGAACTAAGAGCGCT	GCGAAATTTGAGTCACGTGG	20	20	50.0	50.0	58.0	58.0	11.3	17.0	0.065556	65382	65758	91-3000	0.595238	73.5
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	CCGGAATTGCGGGTATTGTA	GCTTTCGAACCAGCATCCTA	20	20	50.0	50.0	58.0	58.0	28.8	15.3	0.067879	65382	65758	91-2276	0.595238	72.9
ycf1_p1	ycf1	1	GCTCGGGTTATGGAAGAAGG	GCTTTTCTTGAACGAAGCCC	20	20	55.0	50.0	58.0	57.9	0.0	8.0	0.096952	108710	108829	91-2278	0.809524	83.0
ycf1_p2	ycf1	2	GCTCGGGTTATGGAAGAAGG	TCTGGTTCTCTTCTCAGCCA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.060529	108710	108829	91-2278	0.666667	75.8
ycf1_p3	ycf1	3	AGGGCCCTCTTATCTCTTCC	TCTGGTTCTCTTCTCAGCCA	20	20	55.0	50.0	57.9	58.0	22.3	0.0	0.126209	108710	108829	92-2278	0.666667	75.2
ycf1_p4	ycf1	4	GGGCCCTCTTATCTCTTCCT	TCTGGTTCTCTTCTCAGCCA	20	20	55.0	50.0	57.9	58.0	17.0	0.0	0.126209	108710	108829	91-2278	0.666667	75.2
ycf1_p5	ycf1	5	AAGGGGGAGAAAGTGAGGAA	TCTGGTTCTCTTCTCAGCCA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.149314	108710	108829	93-2279	0.666667	74.7
ycf1-rps15_p1	ycf1-rps15	1	TGTGTCTGGATGTGATGCTG	CAGCAGTCGTTGACGTTTTC	20	20	50.0	50.0	57.9	58.0	0.0	3.2	0.108725	113508	113797	91-2279	0.928571	88.3
ycf1-rps15_p2	ycf1-rps15	2	TGTGTCTGGATGTGATGCTG	TTCTGGCGCATTTACCGTTA	20	20	50.0	45.0	57.9	57.9	0.0	10.8	0.199757	113508	113797	91-2277	0.928571	87.5
ycf1-rps15_p3	ycf1-rps15	3	TGTGTCTGGATGTGATGCTG	TCCGCCATAAAAGGTCCAAG	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.226320	113508	113797	91-2279	0.928571	87.3
ycf1-rps15_p4	ycf1-rps15	4	TGTGTCTGGATGTGATGCTG	CGCTCTTTGGGGTACTTGAA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.282449	113508	113797	91-2279	0.928571	86.9
ycf1-rps15_p5	ycf1-rps15	5	TGTGTCTGGATGTGATGCTG	AACACCATGCATTGATGGGT	20	20	50.0	45.0	57.9	58.1	0.0	8.9	0.162160	113508	113797	90-2278	0.904762	86.9
ndhF_p1	ndhF	1	AATCCGCACAATTTCCCCTT	GAGCTACTGAGGAACAACGG	20	20	45.0	55.0	58.1	58.0	0.0	0.0	0.065140	126776	126925	93-2281	0.928571	88.6
ndhF_p2	ndhF	2	ACAGCAGGATTAACCGCATT	GCTTCCTTCGTAACTCTCGG	20	20	45.0	55.0	57.9	58.1	0.0	0.0	0.218083	126776	126925	93-2280	0.547619	69.2
ndhF_p3	ndhF	3	ACTTTTGATGGCTGTTTGCG	GCTTCCTTCGTAACTCTCGG	20	20	45.0	55.0	57.9	58.1	0.0	0.0	0.227908	126776	126925	92-2278	0.547619	69.2
ndhF_p4	ndhF	4	GTATGGGGGAGAGAACCACT	GCTTCCTTCGTAACTCTCGG	20	20	55.0	55.0	58.1	58.1	0.0	0.0	0.208372	126776	126925	91-2280	0.523810	68.4
ndhF_p5	ndhF	5	CTGTATGGGGGAGAGAACCA	GCTTCCTTCGTAACTCTCGG	20	20	55.0	55.0	58.1	58.1	0.0	0.0	0.209737	126776	126925	91-2716	0.523810	68.1