Hotspot

rps16

LSC · 5282-6380 · 1099 bp

Back to Diocirea

Pi 0.00303

MarkerSeek score 52.10

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1099
variable_sites: 5
pi: 0.00303
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TGAATTTCGTTTGATTAGACGGA	TCAATTCCATACCGGTTCCA	34.8	45.0	56.6	56.5	1159 bp	1.0	40.3
rps16_p2	TTCGTTTGATTAGACGGAAG	TCAATTCCATACCGGTTCCA	40.0	45.0	53.4	56.5	1154 bp	1.0	40.3
rps16_p3	TGAATTTCGTTTGATTAGACGGA	TCAATTCCATACCGGTTCCAA	34.8	42.9	56.6	57.3	1159 bp	1.0	40.3
rps16_p4	TTTCGTTTGATTAGACGGAAG	TCAATTCCATACCGGTTCCA	38.1	45.0	54.3	56.5	1155 bp	1.0	40.3
rps16_p5	TGAATTTCGTTTGATTAGACGGAA	TCAATTCCATACCGGTTCCA	33.3	45.0	57.2	56.5	1159 bp	1.0	40.3