Hotspot

petD

LSC · 77550-78776 · 1227 bp

Back to Diocirea

Pi 0.0114

MarkerSeek score 44.70

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1227
variable_sites: 21
pi: 0.0114
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	GGAGTTGAAGGGAATTCTCCG	CTTTGAAGCGCCTATTCCCT	52.4	50.0	58.4	58.0	1320 bp	1.0	70.0
petD_p2	GGAGTTGAAGGGAATTCTCCG	TGAAGCGCCTATTCCCTAGA	52.4	50.0	58.4	57.9	1317 bp	1.0	69.7
petD_p3	GAGTTGAAGGGAATTCTCCGA	CTTTGAAGCGCCTATTCCCT	47.6	50.0	57.4	58.0	1319 bp	1.0	68.0
petD_p4	GAGTTGAAGGGAATTCTCCGA	TGAAGCGCCTATTCCCTAGA	47.6	50.0	57.4	57.9	1316 bp	1.0	67.7
petD_p5	TGGAGTTGAAGGGAATTCTCC	CTTTGAAGCGCCTATTCCCT	47.6	50.0	57.3	58.0	1321 bp	1.0	67.2