Hotspot

trnV-GAC-rps12

IRa · 138564-140242 · 1679 bp

Back to Diocirea

Pi 0.00159
MarkerSeek score 60.50
Primer available yes
Label trnV-GAC-rps12

Feature Metadata

label_name
trnV-GAC-rps12
feature_type
igs
region
IRa
length_bp
1679
variable_sites
4
pi
0.00159
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-GAC-rps12_p1 GGGTCGAACTGATGACTTCC TGGCAACTTTACGTAAGGCA 55.0 45.0 58.0 57.7 1768-1856 bp 1.0 78.8
trnV-GAC-rps12_p2 TCAGGGTCGAACTGATGACT TGGCAACTTTACGTAAGGCA 50.0 45.0 58.1 57.7 1771-1859 bp 1.0 78.3
trnV-GAC-rps12_p3 GATGACTTCCACCACGTCAA TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1758-1846 bp 1.0 77.6
trnV-GAC-rps12_p4 AACTGATGACTTCCACCACG TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1762-1850 bp 1.0 77.5
trnV-GAC-rps12_p5 ATGACTTCCACCACGTCAAG TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1757-1845 bp 1.0 77.5