Hotspot

rpl32-trnL-UAG

SSC · 113733-114613 · 881 bp

Back to Diocirea

Pi 0.00832

MarkerSeek score 64.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 881
variable_sites: 11
pi: 0.00832
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGCAGCGTTAAAAGCGTTTT	TTTAGGAGATGACATGCCGC	45.0	50.0	58.5	58.0	1075-1082 bp	1.0	76.3
rpl32-trnL-UAG_p2	AAAGAAGGGGTATTGGGCAG	TTTAGGAGATGACATGCCGC	50.0	50.0	57.5	58.0	1090-1097 bp	1.0	76.2
rpl32-trnL-UAG_p3	GGGTATTGGGCAGCGTTAAA	TTTAGGAGATGACATGCCGC	50.0	50.0	58.5	58.0	1083-1090 bp	1.0	76.2
rpl32-trnL-UAG_p4	GGCAGCGTTAAAAGCGTTTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.5	57.9	1020-1027 bp	1.0	75.7
rpl32-trnL-UAG_p5	AAAGAAGGGGTATTGGGCAG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.5	57.9	1035-1042 bp	1.0	75.6