Hotspot

rps12-trnV-GAC

IRb · 99300-100978 · 1679 bp

Back to Diocirea

Pi 0.00179

MarkerSeek score 60.60

Primer available yes

Label rps12-trnV-GAC

Feature Metadata

label_name: rps12-trnV-GAC
feature_type: igs
region: IRb
length_bp: 1679
variable_sites: 4
pi: 0.00179
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-trnV-GAC_p1	TGGCAACTTTACGTAAGGCA	GGGTCGAACTGATGACTTCC	45.0	55.0	57.7	58.0	1768-1856 bp	1.0	78.8
rps12-trnV-GAC_p2	TGGCAACTTTACGTAAGGCA	TCAGGGTCGAACTGATGACT	45.0	50.0	57.7	58.1	1771-1859 bp	1.0	78.3
rps12-trnV-GAC_p3	TGGCAACTTTACGTAAGGCA	GATGACTTCCACCACGTCAA	45.0	50.0	57.7	57.8	1758-1846 bp	1.0	77.6
rps12-trnV-GAC_p4	TGGCAACTTTACGTAAGGCA	AACTGATGACTTCCACCACG	45.0	50.0	57.7	57.8	1762-1850 bp	1.0	77.5
rps12-trnV-GAC_p5	TGGCAACTTTACGTAAGGCA	ATGACTTCCACCACGTCAAG	45.0	50.0	57.7	57.8	1757-1845 bp	1.0	77.5