Hotspot

trnP-UGG-psaJ

LSC · 67715-68153 · 439 bp

Back to Diocirea

Pi 0
MarkerSeek score 59.50
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
439
variable_sites
0
pi
0
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.95
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 ACCCGTGACTTTTTGTACCC CTAAAGCCCCGAACCATAGC 50.0 55.0 58.0 58.4 515-561 bp 1.0 80.5
trnP-UGG-psaJ_p2 ACCCGTGACTTTTTGTACCC GCTAAAGCCCCGAACCATAG 50.0 55.0 58.0 58.4 516-562 bp 1.0 80.5
trnP-UGG-psaJ_p3 CCCGTGACTTTTTGTACCCA CTAAAGCCCCGAACCATAGC 50.0 55.0 58.0 58.4 514-560 bp 1.0 80.5
trnP-UGG-psaJ_p4 CCCGTGACTTTTTGTACCCA GCTAAAGCCCCGAACCATAG 50.0 55.0 58.0 58.4 515-561 bp 1.0 80.5
trnP-UGG-psaJ_p5 ACAGGATTTGAACCCGTGAC CTAAAGCCCCGAACCATAGC 50.0 55.0 58.1 58.4 526-572 bp 1.0 79.9