Hotspot

petA-psbJ

LSC · 64081-65155 · 1075 bp

Back to Diocirea

Pi 0.000633

MarkerSeek score 61.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1075
variable_sites: 1
pi: 0.000633
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCTGTCATTTTGGCAC	ACTGGTATTCTTGTGCTCGG	50.0	50.0	58.0	57.6	1214-1235 bp	1.0	78.5
petA-psbJ_p2	GGCATCTGTCATTTTGGCAC	CTGGTATTCTTGTGCTCGGT	50.0	50.0	58.0	57.6	1213-1234 bp	1.0	78.5
petA-psbJ_p3	CGAGAAAGTTCAATTGGCCG	ACTGGTATTCTTGTGCTCGG	50.0	50.0	57.8	57.6	1163-1184 bp	1.0	76.6
petA-psbJ_p4	CGAGAAAGTTCAATTGGCCG	CTGGTATTCTTGTGCTCGGT	50.0	50.0	57.8	57.6	1162-1183 bp	1.0	76.6
petA-psbJ_p5	TGGCATCTGTCATTTTGGCA	CTGGTATTCTTGTGCTCGGT	45.0	50.0	58.4	57.6	1214-1235 bp	1.0	75.5