Hotspot

trnT-GGU-psbD

LSC · 32235-33566 · 1332 bp

Back to Diocirea

Pi 0.00426

MarkerSeek score 60.00

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1332
variable_sites: 8
pi: 0.00426
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	55.0	50.0	58.1	58.1	1488-1492 bp	1.0	79.2
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1491-1495 bp	1.0	79.0
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	TCTCCGTAACCAGTCATCCA	55.0	50.0	58.1	57.8	1461-1465 bp	1.0	78.3
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	TCTCCGTAACCAGTCATCCA	50.0	50.0	58.1	57.8	1464-1468 bp	1.0	78.2
trnT-GGU-psbD_p5	GGCGTAAGTCATCGGTTCAA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.3	58.1	1465-1469 bp	1.0	77.5