Hotspot

rps2-rpoC2

LSC · 16796-17014 · 219 bp

Back to Diocirea

Pi 0.0107

MarkerSeek score 47.40

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 219
variable_sites: 3
pi: 0.0107
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	CCGCATCTCTTTTCGGAAGA	GGGTCGAATCGATTGGTTGA	50.0	50.0	58.0	58.0	890-893 bp	1.0	80.4
rps2-rpoC2_p2	CCGCATCTCTTTTCGGAAGA	TATAACCCAGGGGCTTCCAA	50.0	50.0	58.0	58.0	1376-1379 bp	1.0	80.2
rps2-rpoC2_p3	CACCATAGCCGCATCTCTTT	GGGTCGAATCGATTGGTTGA	50.0	50.0	58.0	58.0	898-901 bp	1.0	80.1
rps2-rpoC2_p4	CACCATAGCCGCATCTCTTT	TATAACCCAGGGGCTTCCAA	50.0	50.0	58.0	58.0	1384-1387 bp	1.0	80.0
rps2-rpoC2_p5	TTTCACCATAGCCGCATCTC	TATAACCCAGGGGCTTCCAA	50.0	50.0	58.0	58.0	1387-1390 bp	1.0	79.9