Hotspot

ndhF

SSC · 101934-104153 · 2220 bp

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Pi 0.00826

MarkerSeek score 49.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2220
variable_sites: 27
pi: 0.00826
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGCATTTTGATGAGCCTCGT	TGGGGAGAGAAAACGAAGGT	45.0	50.0	58.2	58.6	2862-2957 bp	1.0	77.8
ndhF_p2	TGCATTTTGATGAGCCTCGT	ATGGGGAGAGAAAACGAAGG	45.0	50.0	58.2	57.2	2863-2958 bp	1.0	76.0
ndhF_p3	TGCATTTTGATGAGCCTCGT	AGGTTTGGTGAATCGGAAACA	45.0	42.9	58.2	58.1	2342 bp	1.0	71.1
ndhF_p4	ATTCTCGAGCCGAAAGATGG	AGGTTTGGTGAATCGGAAACA	50.0	42.9	57.8	58.1	2993 bp	1.0	70.5
ndhF_p5	GAGCCGAAAGATGGATGCAT	AGGTTTGGTGAATCGGAAACA	50.0	42.9	58.4	58.1	2987 bp	1.0	69.1