Hotspot

rpl20-rps12

LSC · 67035-67725 · 691 bp

Back to Dinebra

Pi 0.00485

MarkerSeek score 62.40

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 691
variable_sites: 5
pi: 0.00485
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	ACGCATTTTTGTTCGTCGTC	GAGGAACATGTGCTAGGGTG	45.0	55.0	58.0	58.0	764-768 bp	1.0	80.6
rpl20-rps12_p2	ACGCATTTTTGTTCGTCGTC	GTCGAGGAACATGTGCTAGG	45.0	55.0	58.0	58.1	767-771 bp	1.0	80.3
rpl20-rps12_p3	GTTTGAGGCAAATGAACGCA	GAGGAACATGTGCTAGGGTG	45.0	55.0	57.9	58.0	779-783 bp	1.0	79.6
rpl20-rps12_p4	ACGCATTTTTGTTCGTCGTC	TGCAAGACAGCCAATACGAA	45.0	45.0	58.0	57.8	826-830 bp	1.0	79.3
rpl20-rps12_p5	GTTTGAGGCAAATGAACGCA	GTCGAGGAACATGTGCTAGG	45.0	55.0	57.9	58.1	782-786 bp	1.0	79.3