Hotspot

trnR-UCU-rps14

LSC · 36634-37010 · 377 bp

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Pi 0.0133

MarkerSeek score 62.70

Primer available yes

Label trnR-UCU-rps14

Feature Metadata

label_name: trnR-UCU-rps14
feature_type: igs
region: LSC
length_bp: 377
variable_sites: 7
pi: 0.0133
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-rps14_p1	AGAAGACCTCTGTCCTATCCA	ATGCATGTTTGTTACCGGGT	47.6	45.0	56.9	58.1	454-461 bp	1.0	64.3
trnR-UCU-rps14_p2	AGAAGACCTCTGTCCTATCCA	TCCGGACACATACTTCGAGA	47.6	50.0	56.9	57.9	482-489 bp	1.0	64.0
trnR-UCU-rps14_p3	AGGTTTAGAAGACCTCTGTCCT	ATGCATGTTTGTTACCGGGT	45.5	45.0	57.9	58.1	460-467 bp	1.0	63.6
trnR-UCU-rps14_p4	AGAAGACCTCTGTCCTATCCA	TTACCGGGTGCAACAAGATC	47.6	50.0	56.9	58.2	443-450 bp	1.0	63.6
trnR-UCU-rps14_p5	AGGTTTAGAAGACCTCTGTCCT	TCCGGACACATACTTCGAGA	45.5	50.0	57.9	57.9	488-495 bp	1.0	63.3