Hotspot

trnS-GCU

LSC · 8494-8581 · 88 bp

Back to Dimocarpus

Pi 0.0227

MarkerSeek score 60.30

Primer available yes

Label trnS-GCU

Feature Metadata

label_name: trnS-GCU
feature_type: tRNA
region: LSC
length_bp: 88
variable_sites: 2
pi: 0.0227
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU_p1	AAGATACCAAGCCATCGACG	AGTAAATTAAAGTAAAGAAGGGCT	50.0	29.2	57.8	54.2	189-198 bp	1.0	44.5
trnS-GCU_p2	AAGATACCAAGCCATCGACG	AAGTAAATTAAAGTAAAGAAGGGCT	50.0	28.0	57.8	54.9	190-199 bp	1.0	44.5
trnS-GCU_p3	AAGATACCAAGCCATCGACG	AGTAAATTAAAGTAAAGAAGGGCTT	50.0	28.0	57.8	54.9	189-198 bp	1.0	44.5
trnS-GCU_p4	AAGATACCAAGCCATCGACG	TTAAAGTAAAGAAGGGCTTTTT	50.0	27.3	57.8	52.1	192 bp	0.5	20.0
trnS-GCU_p5	AAGATACCAAGCCATCGACG	ATTAAAGTAAAGAAGGGCTTTTT	50.0	26.1	57.8	52.8	193 bp	0.5	20.0