Hotspot

petB

LSC · 77651-79096 · 1446 bp

Back to Dimocarpus

Pi 0.009

MarkerSeek score 51.90

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1446
variable_sites: 13
pi: 0.009
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	TCGACCGCGAAATTTTTGTG	TAAACGGAATCGATGCAGCA	45.0	45.0	58.0	58.0	2009-2031 bp	1.0	81.5
petB_p2	GATGCATTTGGGTCTCGACT	TAAACGGAATCGATGCAGCA	50.0	45.0	58.0	58.0	2064-2087 bp	1.0	81.5
petB_p3	TCGACCGCGAAATTTTTGTG	GCCGCTGCTCTTCTTTAGAT	45.0	50.0	58.0	58.1	2054-2076 bp	1.0	81.2
petB_p4	TCGACCGCGAAATTTTTGTG	GGTTGTCTCAGCCTTTGTGA	45.0	50.0	58.0	58.0	2182 bp	0.5	59.5
petB_p5	GATGCATTTGGGTCTCGACT	GGTTGTCTCAGCCTTTGTGA	50.0	50.0	58.0	58.0	2238 bp	0.5	59.5