Hotspot

ndhA

SSC · 123924-126110 · 2187 bp

Back to Dimocarpus

Pi 0.00782

MarkerSeek score 52.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2187
variable_sites: 17
pi: 0.00782
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	GGCAAGCCGTGAATATGGTA	TGGGAGAAGTTGATCGTTGA	50.0	45.0	58.0	56.5	2234-2247 bp	1.0	68.7
ndhA_p2	TTTGAGGCAAGCCGTGAATA	TGGGAGAAGTTGATCGTTGA	45.0	45.0	57.8	56.5	2239-2252 bp	1.0	67.5
ndhA_p3	GGCAAGCCGTGAATATGGTA	TGGGAGAAGTTGATCGTTGAA	50.0	42.9	58.0	57.2	2234-2247 bp	1.0	66.7
ndhA_p4	TGAGGCAAGCCGTGAATATG	TGGGAGAAGTTGATCGTTGA	50.0	45.0	58.3	56.5	2237-2250 bp	1.0	66.3
ndhA_p5	TCTCTTGTTTGAGGCAAGCC	TGGGAGAAGTTGATCGTTGA	50.0	45.0	58.4	56.5	2246-2259 bp	1.0	65.9