Hotspot

infA

LSC · 82628-82875 · 248 bp

Back to Dimocarpus

Pi 0.0363

MarkerSeek score 63.30

Primer available yes

Label infA

Feature Metadata

label_name: infA
feature_type: gene
region: LSC
length_bp: 248
variable_sites: 9
pi: 0.0363
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA_p1	GAACCGTGAATTGGATCCTCA	GGGGGTTTACCTGGAATAAAAG	47.6	45.5	58.0	56.6	303 bp	1.0	47.4
infA_p2	TGAACCGTGAATTGGATCCTC	GGGGGTTTACCTGGAATAAAAG	47.6	45.5	58.0	56.6	304 bp	1.0	47.4
infA_p3	ACCGTGAATTGGATCCTCATG	GGGGGTTTACCTGGAATAAAAG	47.6	45.5	58.1	56.6	301 bp	1.0	46.9
infA_p4	GAACCGTGAATTGGATCCTCA	GGGGGTTTACCTGGAATAAA	47.6	45.0	58.0	54.6	303 bp	1.0	46.8
infA_p5	TGAACCGTGAATTGGATCCTC	GGGGGTTTACCTGGAATAAA	47.6	45.0	58.0	54.6	304 bp	1.0	46.8