Hotspot

clpP1

LSC · 72593-74713 · 2121 bp

Back to Dimocarpus

Pi 0.00758

MarkerSeek score 47.50

Primer available yes

Label clpP1

Feature Metadata

label_name: clpP1
feature_type: gene
region: LSC
length_bp: 2121
variable_sites: 16
pi: 0.00758
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1_p1	TGACCCTGTTAGTCCGTTCT	CAATACGCAATGAGGGTCGA	50.0	50.0	58.0	58.1	2946-2953 bp	1.0	80.4
clpP1_p2	ATACACCCGCGTACATGTTC	CAATACGCAATGAGGGTCGA	50.0	50.0	58.1	58.1	2621-2628 bp	1.0	80.0
clpP1_p3	ATACACCCGCGTACATGTTC	ATACCAAGGCAAACCCATGG	50.0	50.0	58.1	58.1	2842-2851 bp	1.0	79.4
clpP1_p4	TGACCCTGTTAGTCCGTTCT	TACGCAATGAGGGTCGATTC	50.0	50.0	58.0	57.8	2943-2950 bp	1.0	79.2
clpP1_p5	ATACACCCGCGTACATGTTC	CGGAGGGATCAAAAACAGCT	50.0	50.0	58.1	58.2	2969-2978 bp	1.0	79.1