Hotspot

atpA

LSC · 10281-11804 · 1524 bp

Back to Dimocarpus

Pi 0.00262

MarkerSeek score 60.20

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 4
pi: 0.00262
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	GGTTCAAATCCTATTGGACGC	ATTGACACGTTTGAGGCGAT	47.6	45.0	57.3	58.2	1718-1889 bp	1.0	65.1
atpA_p2	GGTTCAAATCCTATTGGACGC	TATTGACACGTTTGAGGCGA	47.6	45.0	57.3	57.6	1719-1890 bp	1.0	63.1
atpA_p3	GGTTCAAATCCTATTGGACGC	CGTTTGAGGCGATGGAAGAA	47.6	50.0	57.3	58.6	1711-1882 bp	1.0	62.1
atpA_p4	GTTCAAATCCTATTGGACGCA	ATTGACACGTTTGAGGCGAT	42.9	45.0	56.6	58.2	1717-1888 bp	1.0	59.5
atpA_p5	GGTTCAAATCCTATTGGACGC	TATTGACACGTTTGAGGCGAT	47.6	42.9	57.3	58.0	1719-1890 bp	1.0	58.4