Hotspot

rps15-ycf1

SSC · 127698-128062 · 365 bp

Back to Dimocarpus

Pi 0.022

MarkerSeek score 54.20

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 365
variable_sites: 8
pi: 0.022
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	GGATAGTAACCGTTGGCGTT	CCGCTTCGTCTGTCTGTAAA	50.0	50.0	58.0	57.9	1086-1094 bp	1.0	81.0
rps15-ycf1_p2	AACACCATGCATTGATGGGT	CCGCTTCGTCTGTCTGTAAA	45.0	50.0	58.1	57.9	1355-1363 bp	1.0	80.4
rps15-ycf1_p3	CAACAGACCCGCTCTTTTCT	CCGCTTCGTCTGTCTGTAAA	50.0	50.0	58.1	57.9	958-966 bp	1.0	80.4
rps15-ycf1_p4	TCAACAGACCCGCTCTTTTC	CCGCTTCGTCTGTCTGTAAA	50.0	50.0	58.1	57.9	959-967 bp	1.0	80.4
rps15-ycf1_p5	TTTCCGTAGACCCCTCTGAG	CCGCTTCGTCTGTCTGTAAA	55.0	50.0	58.2	57.9	1056-1064 bp	1.0	80.0