Hotspot

ccsA-ndhD

SSC · 119602-119911 · 310 bp

Back to Dimocarpus

Pi 0.0465

MarkerSeek score 67.60

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 310
variable_sites: 14
pi: 0.0465
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	AACCGTCGGTATTCTTTCGG	CGGCAAATGTTCTACGGGTA	50.0	50.0	58.0	58.0	770-793 bp	1.0	82.5
ccsA-ndhD_p2	AACCGTCGGTATTCTTTCGG	TACGGCAAATGTTCTACGGG	50.0	50.0	58.0	58.0	772-795 bp	1.0	82.4
ccsA-ndhD_p3	AACCGTCGGTATTCTTTCGG	TCAATGGCTTCCCTTGCATT	50.0	45.0	58.0	58.1	938-961 bp	1.0	81.7
ccsA-ndhD_p4	TACCCAAGGCTTTGCAACTT	CGGCAAATGTTCTACGGGTA	45.0	50.0	57.9	58.0	1163-1186 bp	1.0	81.2
ccsA-ndhD_p5	AACCGTCGGTATTCTTTCGG	GAACTATTACCGCACGCTCA	50.0	50.0	58.0	58.1	1283-1306 bp	1.0	81.0