Hotspot

rpl33-rps18

LSC · 70461-70662 · 202 bp

Back to Dimocarpus

Pi 0.0352

MarkerSeek score 60.40

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 202
variable_sites: 7
pi: 0.0352
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	ACCAGTTGTGTTCGAAACGA	GCAAACGCCTACGAAAAGAC	45.0	50.0	58.0	58.1	419-422 bp	1.0	81.8
rpl33-rps18_p2	ACCAGTTGTGTTCGAAACGA	AACGCCTACGAAAAGACCTC	45.0	50.0	58.0	57.9	416-419 bp	1.0	81.6
rpl33-rps18_p3	GGTTCATGGCCAAGGGTAAA	GCAAACGCCTACGAAAAGAC	50.0	50.0	58.1	58.1	469-472 bp	1.0	81.2
rpl33-rps18_p4	AAAGGAGGTTCATGGCCAAG	GCAAACGCCTACGAAAAGAC	50.0	50.0	58.1	58.1	475-478 bp	1.0	81.2
rpl33-rps18_p5	GGTTCATGGCCAAGGGTAAA	AACGCCTACGAAAAGACCTC	50.0	50.0	58.1	57.9	466-469 bp	1.0	81.1