Hotspot

trnW-CCA-trnP-UGG

LSC · 69010-69200 · 191 bp

Back to Dimocarpus

Pi 0.0317

MarkerSeek score 67.00

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 191
variable_sites: 6
pi: 0.0317
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	GTTTTGGAGACCCACGTTCT	GGGTACAAAATGTCACGGGT	50.0	50.0	58.0	58.1	269-271 bp	1.0	81.3
trnW-CCA-trnP-UGG_p2	GTTTTGGAGACCCACGTTCT	TGGGTACAAAATGTCACGGG	50.0	50.0	58.0	58.1	270-272 bp	1.0	81.3
trnW-CCA-trnP-UGG_p3	GTTTTGGAGACCCACGTTCT	GTCACGGGTTCAAATCCTGT	50.0	50.0	58.0	58.1	258-260 bp	1.0	81.3
trnW-CCA-trnP-UGG_p4	GTTTTGGAGACCCACGTTCT	CACGGGTTCAAATCCTGTCA	50.0	50.0	58.0	58.1	256-258 bp	1.0	81.3
trnW-CCA-trnP-UGG_p5	GTTTTGGAGACCCACGTTCT	TGTCACGGGTTCAAATCCTG	50.0	50.0	58.0	58.1	259-261 bp	1.0	81.3