Hotspot

petG-trnW-CCA

LSC · 68798-68935 · 138 bp

Back to Dimocarpus

Pi 0.0547

MarkerSeek score 79.90

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 138
variable_sites: 7
pi: 0.0547
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	CAATACAGGCGTGGTGATCA	AGAACGTGGGTCTCCAAAAC	50.0	50.0	58.0	58.0	221-224 bp	1.0	85.6
petG-trnW-CCA_p2	ACAATACAGGCGTGGTGATC	AGAACGTGGGTCTCCAAAAC	50.0	50.0	58.0	58.0	222-225 bp	1.0	85.6
petG-trnW-CCA_p3	CAATACAGGCGTGGTGATCA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	243-246 bp	1.0	85.3
petG-trnW-CCA_p4	ACAATACAGGCGTGGTGATC	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.0	58.0	244-247 bp	1.0	85.2
petG-trnW-CCA_p5	CGGGATTATTCGTAACCGCA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.1	58.0	269-272 bp	1.0	83.7