Hotspot

petA-psbJ

LSC · 65532-66289 · 758 bp

Back to Dimocarpus

Pi 0.016

MarkerSeek score 58.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 758
variable_sites: 12
pi: 0.016
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGAGAAGGTTCAATTGTCCGA	GGATTGGGTTCGTCGTTGTA	42.9	50.0	57.2	57.9	806-813 bp	1.0	67.2
petA-psbJ_p2	TGAGAAGGTTCAATTGTCCGAA	GGATTGGGTTCGTCGTTGTA	40.9	50.0	57.9	57.9	806-813 bp	1.0	64.7
petA-psbJ_p3	TTGAGAAGGTTCAATTGTCCGA	GGATTGGGTTCGTCGTTGTA	40.9	50.0	57.9	57.9	807-814 bp	1.0	64.7
petA-psbJ_p4	TGAGAAGGTTCAATTGTCCGA	TATTCCGGATTGGGTTCGTC	42.9	50.0	57.2	57.4	812-819 bp	1.0	63.0
petA-psbJ_p5	TGAGAAGGTTCAATTGTCCG	GGATTGGGTTCGTCGTTGTA	45.0	50.0	55.7	57.9	806-813 bp	1.0	62.8